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Quantification of the effect of factors involved in challenge-test assays on the growth rate estimation of Listeria monocytogenes.<br /><br /><br /><br /><br />Quantification des effets de facteurs lors de la réalisation de tests de croissance aliments sur l’estimation du taux de croissance de Listeria monocytogenes.

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Direct evaluation of growth of artificially inoculated bacterial pathogens in foods using challenge-tests is an interesting tool for the management of food safety.

However, an issue is their an issue is their ability to describe the growth of a foodborne pathogen in conditions as similar as possible to a naturally contamined and routinely produced food.
PDF icon Quantification of the effect of factors involved in challenge-test assays on the growth rate estimation of Listeria monocytogenes.<br /><br /><br /><br /><br />Quantification des effets de facteurs lors de la réalisation de tests de croissance aliments sur l’estimation du taux de croissance de Listeria monocytogenes.
2010

Design of challenge testing experiments to assess the variability of microbial behaviors in foods

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The assessment of the evolution of microorganisms naturally contaminating food must take into account the variability of biological factors, food characteristics and storage conditions. A research project involving eight French laboratories was conducted to quantify the variability of growth parameters of Listeria monocytogenes obtained by challenge testing in five foods. The residual variability corresponded to a coefficient of variation (CV) of approximately 20% for the growth rate (μmax) and 120% for the parameter K (=μmax.lag time).
PDF icon Design of challenge testing experiments to assess the variability of microbial behaviors in foods
2009

Modelling microbial competition in foods. Application to the behaviour of Listeria monocytogenes and lactic acid flora in diced bacon

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The current models developed in predictive microbiology to describe interactions between microflora in foods are reviewed, with a special focus on the Jameson-effect and Lotka- Volterra approaches. One case-study is further explored: modelling the sparse growth of Listeria monocytogenes in diced bacon along the shelf-life.
PDF icon Modelling microbial competition in foods. Application to the behaviour of Listeria monocytogenes and lactic acid flora in diced bacon
2009

Quantification of the effect of factors involved in challenge-test assays on the growth rate estimation of Listeria monocytogenes

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This study describes an original interlaboratory trial relative to food challenge-tests conducted by eight French laboratories. The impact of several factors (linked to the type of foodstuff, to biological parameters and to experimental conditions as well as to the laboratory performing the test) on the growth rate of Listeria monocytogenes was quantified. The major factors influencing the growth rate variability were the "manufacturing origin of the product" and the "localization of the inoculum in food".
PDF icon Quantification of the effect of factors involved in challenge-test assays on the growth rate estimation of Listeria monocytogenes
2009

Sampling plan optimisation : application to French diced bacon industry and Listeria monocytogenes

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We designed an experimental sampling plan to get knowledge on L. monocytogenes and on lactic acid bacteria (LAB) concentrations on pork breasts after tumbling in a French diced bacon plant. Then, we built a Bayesian model to estimate variability (including between batches-variability) and uncertainty. The results of the model are used to predict L. monocytogenes concentration on breasts after tumbling and could be useful to optimise a sampling plan for industrial own-checks.
PDF icon Sampling plan optimisation : application to French diced bacon industry and Listeria monocytogenes
2009

Probabilistic modeling of listeria monocytogenes behaviour in diced bacon along the manufacture process chain

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To assess the impact of the manufacturing process on the fate of L. monocytogenes, we built a probabilistic model describing successively the different steps of the process. The model was actually designed as a hierarchical Bayesian network leading to the elicitation of human expertise. Contamination evolution was modelled in the adequate units (breasts, dices, then packaging units through the successive process steps). The use of probabilistic modeling allowed taking into account both the process intrinsic variability and parameter variability or uncertainty.
PDF icon Probabilistic modeling of listeria monocytogenes behaviour in diced bacon along the manufacture process chain
2009

Incidence du mode de conditionnement sur la durée de vie de la viande de porc en UVCI

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Cette étude propose une démarche originale afin d'évaluer l'impact du conditionnement sur l'altération microbiologique de côtes de porc en UVCI.La comparaison et le suivi de deux modes de conditionnement (sous film et sous atmosphère) a permis de caractériser le comportement de flores d'altérations et d'extrapoler les résultats pour d'autres scenarii thermiques que celui testé expérimentalement grâce au logiciel Sym'Previus.
PDF icon Incidence du mode de conditionnement sur la durée de vie de la viande de porc en UVCI
2009

Sym'Previus : des assistants informatiques pour estimer l'exposition du consommateur aux dangers microbiens.

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La croissance bactérienne est influencée par des paramètres biologiques (contamination initiale, valeurs cardinales, taux de croissance optimum), par les caractéristiques de l'aliment (pH, aW, etc.) et par les conditions de stockage. Dans les conditions naturelles, ces paramètres et facteurs ne sont pas figés, mais au contraire, sont parfois extrêmement variables (variabilité de la contamination initiale, des souches, du pH et de l'aW, des conditions de conservation, etc).
PDF icon Sym'Previus : des assistants informatiques pour estimer l'exposition du consommateur aux dangers microbiens.
2008

Optimising food process and formulation through Sym'Previus food safety management

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Sym’Previus network gathers expertises in predictive microbiology from major food companies, French technical centres and public research institutes. Based on the most recent concepts in predictive microbiology, Sym’Previus proposes an assistance in food safety management.

Sym’Previus predictive tool is composed of a database and an advanced simulation software.
PDF icon Optimising food process and formulation through Sym'Previus food safety management
2007

Integrated modelling of food process and bacterial behaviour : application for predicting the evolution of Listeria monocytogenes contamination during delicatessen processing

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Microorganism, food and process are the three main components involved in the quality and safety of food products. Indeed food products may become microbiologically unsafe if the growth of pathogenic or spoilage bacteria occurred in particular when they are not properly processed and stored. Predictive microbiology is a powerful tool that can help professional to guarantee the microbiological quality of their products. Nevertheless, too many models have been built in static condition with broth medium.
PDF icon Integrated modelling of food process and bacterial behaviour : application for predicting the evolution of Listeria monocytogenes contamination during delicatessen processing
2007

Growth probability of Listeria monocytogenes and classification of pork meat products

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The performance of a growth probability model taking into account the pH, the water activity, and the storage temperature of food to predict the growth or the no growth of Listeria monocytogenes in pork meat products was assessed by comparing predictions with the behaviour observed in these products by challenge-testing. The predictive positive value of the model was 100% for cooked pork products characterized by high pH/water activity combinations and that always supported the growth of L. monocytogenes.
PDF icon Growth probability of Listeria monocytogenes and classification of pork meat products
2007

Prediction of the evolution of the contamination of Listeria monocytogenes during the manufacturing and the storage of delicatessen : combined modelling of heat tranfer and bacterial behaviour

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PDF icon Prediction of the evolution of the contamination of Listeria monocytogenes during the manufacturing and the storage of delicatessen : combined modelling of heat tranfer and bacterial behaviour
2007

Modelling the behaviour of Listeria monocytogenes in ground pork as a function of pH, water activity, nature and concentration of organic acid salts

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Aims : to study and model the effect of sodium acetate, sodium lactate, potassium sorbate and combination of acid salts on the behaviour of Listeria monocytogenes in ground pork.


2007

Prévision de l'évolution de contamination par Listeria monocytogenes de produits de salaison lors de leur fabrication et de leur conservation : modélisation combinée des transferts thermiques et des cinétiques bactériennes

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PDF icon Prévision de l'évolution de contamination par Listeria monocytogenes de produits de salaison lors de leur fabrication et de leur conservation : modélisation combinée des transferts thermiques et des cinétiques bactériennes
2007

Effets du type de traitement thermique, du pH, de l'activité de l'eau et de leurs interactions sur le comportement de Listeria monocytogenes dans la viande hachée de porc.

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Modelling the boundary of the growth/no-growth areas as a function of pH, water activity and temperature.

The influence of four heat-processing regimes and a storage phase on the behaviour of Listeria monocytogenes in ground pork was studied. The effects of pH and water activity (aw) were also tested.

During the heat process phase, aw, the heat-processing regime and its interactions with pH or aw, had a significant effect on the behaviour of L. monocytogenes. During the storage phase, all parameters tested and their interactions had significant effects. Nevertheless, the area in which the growth of L. monocytogenes was observed at the end of the experiment was not influenced by the heat-processing regime tested. On the contrary, pH, aw and their interactions had significant effects on Listeria behaviour. The boundary of the growth area delimited by environmental conditions where growth was higher than 1.0 Log CFU g)1 from those where growth was lower than this limit was correctly predicted by Augustin’s model.

2006

Microbiologie prévisionnelle et sécurité des aliments

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Les professionnels de l’agro-alimentaire doivent aujourd’hui apporter la preuve de la qualité et de la sécurité de leurs produits. En effet, l'ouverture des marchés impose aux entreprises des exigences sévères en matière d'hygiène des aliments. Afin de répondre à ces exigences, plusieurs outils permettent d'évaluer les risques de développement microbien et les durées de vie des produits.

PDF icon Microbiologie prévisionnelle et sécurité des aliments
2006

Prédiction de la contamination bactérienne lors de la fabrication et de la conservation d’un aliment - Application à de la viande de porc contaminée par Listeria monocytogenes

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En France, les récentes épidémies de listériose ont conduit les professionnels de la charcuterie/salaison à étudier les potentialités de croissance, de survie et de destruction de Listeria monocytogenes, au cours de la fabrication et de la conservation de leurs produits. La microbiologie prévisionnelle est un outil qui peut les aider à atteindre cet objectif. Dans ce travail, un modèle a été développé pour prédire l’évolution de contamination de L. monocytogenes, dans de la viande de porc, soumise au procédé de fabrication des

Document réservé Espace Pro, veuillez vous identifier
2005

Etude et modélisation du développement de Listeria monocytogenes dans la viande de porc en présence de sels d'acides organiques et de fumée liquide - Application pour prédire l'influence de la formulation de la saumure et de la fumée de fumage lors du pro

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Application pour prédire l’influence de la formulation de la saumure et de la fumée de fumage lors du procédé de fabrication des lardons.
Les récentes épidémies de listériose ont conduit les professionnels de la charcuterie/salaison à étudier les potentialités de croissance et de survie de Listeria monocytogenes au cours de la fabrication de leurs produits. Dans cet objectif, la microbiologie prévisionnelle est un outil développé pour les aider.

Document réservé Espace Pro, veuillez vous identifier
2005

L'évaluation de la qualité microbiologique de la viande de porc, matière première du saucisson sec (Partie I)

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Cette première partie dresse un inventaire des éléments disponibles pour l'évaluation de la qualité microbiologique de la viande de porc destinée à la fabrication de saucisson sec : définition du critère microbiologique selon le Codex Alimentarius (Alinorm 97 / 13), analyse de risques (Codex Alimentarius, 1995), réglementation (arrêté du 21 décembre 1979, note de service du 12 mai 1998, arrêté du 22 janvier 1993), guides de bonnes pratiques d'hygiène (1994, 2000), microbiologie prévisionnelle (1995), HACCP (Codex Alimentarius, 1993), assurance qualité (approche ISO9002, 1987, 1994, 2000).
PDF icon L'évaluation de la qualité microbiologique de la viande de porc, matière première du saucisson sec (Partie I)
2002

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