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Second harmonic generation microscopy of pse-like zones from pork semimembranosus

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POSTER. Histological investigations of the PSE-like zones are not frequent in the bibliography, most of the studies are focused on the infl uence of the main risk factors of this ham’s defect. The aim of this work is to test the feasability of a SHG (Second Harmonic Generation) microscopy study of PSE-like zones. SHG microscopy is a rapid tool (fast sample preparation) that allows the three-dimensional study of skeletal muscle.

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2013

Second harmonic generation microscopy of pse-like zones from pork semimembranosus

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The aim of this work was to study the sarcomeric organization of muscles with PSE-like zones and normal muscle (control), focusing on the SHG signal pattern of myosin (single or double band). Second harmonic generation microscopy has been applied on pork Semimembranosus samples fixed at 24 hours post mortem. Electronic microscopy was applied on the same to improve SHG images interpretation. PSE-like zones were presenting SHG signla with a higher single band frequency than the control muscles (87.5% vs 35%, respectively).

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2013

Microstructure des émulsions et dispersions alimentaires (ACTIA RA 96-08)

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2001