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Salivary and urinary metabolome analysis for pre-puberty-related biomarkers identification in porcine

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Ghylène Goudet et al., Animal, 2019, volume 13, n° 4, avril, p. 760-770

Estrus synchronization is important for optimal management of gilt reproduction in pig farms. Hormonal treatments, such as synthetic progestogens, are used on a routine basis, but there is a growing demand for non-hormonal alternative breeding tools. Before puberty, gilts exhibit a ‘waiting period,’ related to the ovarian development and gonadotrophin secretions, during which external stimulations, such as boar exposure, could induce and synchronize first ovulation. Practical non-invasive tools for identification of this period in farms are lacking. During this period, urinary oestrone levels are high, but urine sampling is difficult in group-housed females. The aim of this work was to search for specific biomarkers of the ‘waiting period’ in saliva and urine. In total, nine 144- to 147-day-old Large White gilts were subjected to trans-abdominal ultrasonography three times a week for 5 weeks until puberty detection (week –5 to week –1 before puberty). Urine and saliva samples were collected for oestrone assay to detect the ‘waiting period’ and for metabolome analysis using 1H-nuclear magnetic resonance spectroscopy to detect potential biomarkers of the ‘waiting period.’ Gilts were slaughtered 7 days after puberty detection for puberty confirmation. Results were consistent with ultrasonography data for six gilts. Urine and saliva samples from these six gilts were analyzed. Urinary estrone concentration significantly increased 2 weeks before puberty detection. Metabolome analysis of urine samples allowed the identification of 78 spectral bins, among them, 42 low-molecular-weight metabolites were identified. Metabolome analysis of salivary samples allowed the identification of 59 spectral bins, among them, 23 low-molecular-weight metabolites were detected and 17 were identified. No potential biomarker was identified in urinary samples. In saliva, butyrate and 2HOvalerate, 5.79 ppm (putatively uridine), formate, malonate and propionate could be biomarker candidates to ascertain the pre-puberty period in gilt reproduction. These results confirm that non-invasive salivary samples could allow the identification of the physiological status of the gilts and presumably the optimal time for application of the boar effect. This could contribute to synchronize puberty onset and hence to develop non-hormonal breeding tools.

https://www.cambridge.org/core/services/aop-cambridge-core/content/view/FA6DFF10CB490FA7F83C89B8F3696779/S1751731118002161a.pdf/salivary_and_urinary_metabolome_analysis_for_prepubertyrelated_biomarkers_identification_in_porcine.pdf

2019

Salivary and urinary metabolome analysis for pre-puberty-related biomarkers identification in porcine

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Gylène Gaudet et al., Animal, 2018, 5 septembre, 11 pages

Estrus synchronization is important for optimal management of gilt reproduction in pig farms. Hormonal treatments, such as synthetic progestogens, are used on a routine basis, but there is a growing demand for non-hormonal alternative breeding tools. Before puberty, gilts exhibit a 'waiting period,' related to the ovarian development and gonadotrophin secretions, during which external stimulations, such as boar exposure, could induce and synchronize first ovulation. Practical non-invasive tools for identification of this period in farms are lacking. During this period, urinary oestrone levels are high, but urine sampling is difficult in group-housed females. The aim of this work was to search for specific biomarkers of the 'waiting period' in saliva and urine. In total, nine 144- to 147-day-old Large White gilts were subjected to trans-abdominal ultrasonography three times a week for 5 weeks until puberty detection (week -5 to week -1 before puberty). Urine and saliva samples were collected for oestrone assay to detect the 'waiting period' and for metabolome analysis using 1H-nuclear magnetic resonance spectroscopy to detect potential biomarkers of the 'waiting period.' Gilts were slaughtered 7 days after puberty detection for puberty confirmation. Results were consistent with ultrasonography data for six gilts. Urine and saliva samples from these six gilts were analyzed. Urinary estrone concentration significantly increased 2 weeks before puberty detection. Metabolome analysis of urine samples allowed the identification of 78 spectral bins, among them, 42 low-molecular-weight metabolites were identified. Metabolome analysis of salivary samples allowed the identification of 59 spectral bins, among them, 23 low-molecular-weight metabolites were detected and 17 were identified. No potential biomarker was identified in urinary samples. In saliva, butyrate and 2HOvalerate, 5.79 ppm (putatively uridine), formate, malonate and propionate could be biomarker candidates to ascertain the pre-puberty period in gilt reproduction. These results confirm that non-invasive salivary samples could allow the identification of the physiological status of the gilts and presumably the optimal time for application of the boar effect. This could contribute to synchronize puberty onset and hence to develop non-hormonal breeding tools.

https://www.cambridge.org/core/services/aop-cambridge-core/content/view/FA6DFF10CB490FA7F83C89B8F3696779/S1751731118002161a.pdf/salivary_and_urinary_metabolome_analysis_for_prepubertyrelated_biomarkers_identification_in_porcine.pdf

2018

Conditions de mise en oeuvre et intérêt du contrôle échographique de puberté en élevage porcin

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L’intérêt du contrôle échographique de puberté est évalué pour la 1ère fois dans des conditions d’élevage et avec du matériel de qualité standard. Les mesures ont été réalisées par voie externe, sur 63 cochettes âgées de 180 à 200 jours. L’exactitude des contrôles effectué, avec un appareil portable équipé d’une sonde linéaire, est comparable à celle obtenue avec des appareils de qualité médicale.
PDF icon Conditions de mise en oeuvre et intérêt du contrôle échographique de puberté en élevage porcin
2006

L'échographie, un outil fiable pour détecter la puberté chez la truie

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2006

Ultrasonographic field evaluation of puberty using portable standard equipment

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2006

Conditions de mise en oeuvre et intérêt du contrôle échographique de puberté en élevage porcin

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L’intérêt du contrôle échographique de puberté est évalué pour la première fois dans des conditions d’élevage et avec
PDF icon Conditions de mise en oeuvre et intérêt du contrôle échographique de puberté en élevage porcin
2006

Facteurs de variation de l’âge à la puberté, du taux d’ovulation et de la survie embryonnaire précoce chez la cochette : type génétique, poids vif, saison et consanguinité

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Les paramètres de reproduction de truies nullipares Large White, Large White hyperprolifique et Meishan utilisées comme donneuses de jeunes embryons (jour 5 à 11 de gestation) produits à l’élevage expérimental de la station de Physiologie de la Reproduction de Nouzilly ont été analysés entre octobre 1994 et octobre 1996; ils concernent l’âge à la puberté, le taux d’ovulation, le nombre d’embryons et la survie embryonnaire très précoce; ils ont été comparés à ceux publiés antérieurement pour le même troupeau.
PDF icon Facteurs de variation de l’âge à la puberté, du taux d’ovulation et de la survie embryonnaire précoce chez la cochette : type génétique, poids vif, saison et consanguinité
1997